Ponceau S

Ponceau S Acid Red 112 or C.I. 27195 is a sodium salt of a diazo dye of a light red color that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting) as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (wv) Ponceau S in 5% acetic acid or 2% (wv) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.

For R&D use onlynot for drug household or other uses.