22-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)

22-Azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay). ABTS is significantly used by the food industry to measure the antioxidant capacities within different foods. ABTS is also used to monitor glucose concentrations in different solutions including blood serum. It is recommended for ELISA (microwell) procedures not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results. 22-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures not recommended for membrane applications.

For R&D use onlynot for drug household or other uses.

ABTS 22-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is water soluble chemical compound used as chromogenic substrate ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example). ABTS together with laccase or bilirubin oxidase. A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). ABTS 22-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.