Used in proteomics to deblock N-terminal pyroglutamate for Edman sequencing protein sequencing analysis and removal of pGlu blocking groups prior to mass spectrometry.

Enables N-terminal sequence analysis of proteinspeptides blocked by pyroglutamate formation particularly useful for therapeutic peptides and post-translational modification studies.

Pyroglutamate Aminopeptidase from Pyrococcus furiosus recombinant and expressed in E. coli is a thermostable enzyme (90% purity by SDS-PAGE 5.0 unitsmg protein) with molecular weight 24-28 kDa that specifically hydrolyzes N-terminal pyroglutamic acid (pGlu) residues from peptides and proteins.