0.5 M DCI Solution (4, 5-Dicyanoimidazole in Anhydrous Acetonitrile) PurOligo

PurOligo


Linear Formula:  C5H2N4
Molecular Weight:  118.10 g/mol

Properties

Vapour pressure 97 hPa
Water Solubility Soluble
Explosion limit Upper explosion limit: 16 %(V) Lower explosion limit: 3 %(V)
B pt. 81°C
M pt. -46°C
Form Liquid

Safety Information

Hazard Statement(s) H225-H302+H312+H332-H315-H319-H335
Precautionary Statement  P210-P280-P303+P361+P353-P304+P340-P312-P305+P351+P338
GHS Pictogram
Signal word Danger
HS Code 29332900
Flash point 2°C
Storage Temp. Room Temperature
Storage Class 3 - Flammable liquids
UN Number 1993

Description

Other Notes

45-Dicyanoimidazole (DCI) is an efficient activator for use in chemical synthesis of DNA.

Application
it is used as an activator in automated solid-phase DNA and RNA oligonucleotide synthesis particularly during the phosphoramidite coupling step.

Purpose
Its purpose is to activate nucleoside phosphoramidites making them sufficiently reactive to couple with the free hydroxyl group of the growing oligonucleotide chain and thereby facilitate efficient chain elongation.

General Description
The solution contains 45-dicyanoimidazole (DCI) at a concentration of 0.5 M dissolved in anhydrous acetonitrile. DCI functions as a mild acidic activator that promotes the coupling reaction between the phosphoramidite and the growing oligonucleotide.