0.25 M DCI Solution (4, 5-Dicyanoimidazole in Anhydrous Acetonitrile) PurOligo

PurOligo


Properties

Vapour pressure 97 hPa
Water Solubility Soluble
Explosion limit Upper explosion limit: 16 %(V) Lower explosion limit: 3 %(V)
B pt. 81°C
M pt. -46°C
Form Liquid

Safety Information

Hazard Statement(s) H225-H302+H312+H332-H315-H318-H335
Precautionary Statement  P210-P280-P303+P361+P353-P304+P340-P312-P305+P351+P338
GHS Pictogram
Signal word Danger
HS Code 29339990
Flash point 2°C
Storage Temp. Room Temperature
Storage Class 3 - Flammable liquids
UN Number 1993

Description

Other Notes

45-Dicyanoimidazole (DCI) is an efficient activator for use in chemical synthesis of DNA.

Application
It is used as an activator solution during automated solid-phase synthesis of DNA and RNA oligonucleotides.

Purpose
Its main purpose is to activate phosphoramidites so that they can efficiently react with the growing oligonucleotide chain during the coupling step.

General Description
The solution contains 45-dicyanoimidazole (DCI) at a concentration of 0.25 M dissolved in anhydrous acetonitrile. DCI acts as a mild acidic activator and promotes the coupling of nucleoside phosphoramidites with the reactive hydroxyl group on the growing oligonucleotide chain.