0.3 M 5-Benzylmercaptotetrazole (BMT) in Acetonitrile with 0.5% N-Methylimidazole (NMI) PurOligo [Activator]
PurOligo
Properties
| Assay Purity | 5-Benzylmercaptotetrazole: > 99 %, Acetonitrile : > 99.9 %, N-Methylimidazole : > 99.9 % |
| Form | Liquid |
Safety Information
| Hazard Statement(s) | H225-H302+H312+H332-H319 |
| Precautionary Statement | P210-P280-P301+P312-P303+P361+P353-P304+P340-P312-P305+P351+P338 |
| GHS Pictogram |
|
| Signal word | Danger |
| HS Code | NA |
| Flash point | 2°C |
| Storage Temp. | Room Temperature |
| Storage Class | 3 - Flammable liquids |
| UN Number | 1993 |
Description
Application
it is used as an activator solution in solid-phase DNA and RNA oligonucleotide synthesis particularly in phosphoramidite coupling reactions.
Purpose
Its purpose is to activate nucleoside phosphoramidites by facilitating the reaction between the phosphoramidite and the free hydroxyl group of the growing oligonucleotide chain thereby promoting efficient and rapid chain elongation.
General Description
The solution contains 5-benzylmercaptotetrazole (BMT) at 0.3 M concentration dissolved in acetonitrile with 0.5% N-methylimidazole (NMI) added as a co-component. BMT acts as an effective acidic activator for phosphoramidite coupling while acetonitrile serves as the anhydrous organic solvent and NMI helps support the activation and coupling reaction.
Documents
| SDS | |
| COA | |
| Specification | |
| Bulk quote order form | |
| Sample COA |
