Protein chemistry- Used to label amino acids especially in N-terminal analysis of proteins (classic Sangers reagent). Organic synthesis- Helps in nucleophilic aromatic substitution reactions. Analytical chemistry- Used to derivatize amines for detection and study.

Acts as a derivatizing agent for amines allowing identification or modification of amino groups in molecules (like amino acids and proteins).

An aromatic compound with one fluorine and two nitro (-NO2) groups on a benzene ring. The strong electron-withdrawing nitro groups make it highly reactive toward nucleophiles.