It is widely used as an ion-pairing reagent in high-performance liquid chromatography (HPLC) to aid in the separation of polar and charged analytes. It is also applied in analytical and pharmaceutical laboratories.

The purpose of this compound is to improve chromatographic separation by increasing the retention of ionic substances thereby enhancing the precision and reliability of analytical results.

1-Hexanesulfonic acid sodium salt anhydrous is a white water-soluble ionic compound with the chemical formula CHSONa. It is an anionic surfactant and lacks water of crystallization making it more concentrated than its hydrated form.