It is primarily used in high-performance liquid chromatography (HPLC) as an ion-pairing agent to improve the separation of polar and ionic compounds. It is also utilized in biochemical and pharmaceutical analysis.

The purpose of this compound is to enhance retention and resolution of analytes in chromatographic systems by forming ion pairs thereby improving the accuracy and efficiency of chemical analysis.

1-Hexanesulfonic acid sodium salt monohydrate is an anionic surfactant and ion-pairing reagent with the chemical formula CHSONaHO. It appears as a white crystalline powder that is highly soluble in water and commonly used in analytical chemistry.