Oligonucleotide synthesis It acts as a key reagent in the chemical synthesis of DNA and RNA especially in the phosphoramidite method. Phosphitylation reactions Used to introduce protected phosphate groups into nucleosides. Biotechnology & research Essential in making primers probes and modified nucleic acids for PCR sequencing and gene studies.

The reagent activates hydroxyl groups for phosphite triester formation which oxidizes to stable phosphate linkages post-synthesis its cyanoethyl group acts as a removable protecting group under basic conditions.

2-Cyanoethyl NN-diisopropylchlorophosphoramidite is a synthetic organophosphorus reagent used in nucleic acid chemistry. It appears as a colorless to pale yellow liquid at room temperature.