It serves as a certified reference material for HPLC method validation stability testing and quality control during Rifampicin (anti-TB drug) production particularly for ANDA submissions

Its primary purpose is analytical impurity profiling to ensure Rifampicin purity complies with regulatory limits (0.5% total impurities) aiding detection via chromatography

25-Desacetyl-27-demethyl Rifampicin (CHNO) is a Rifampicin impurity and degradation product lacking both the 25-acetyl group and 27-methyl group from the parent antibiotic rifampicin appearing as a solid used as a reference standard in pharmaceutical analysis with molecular weight 766.90 gmol.