Fluorescent tracer in biochemical and analytical experiments. Indicator in chromatography (especially TLC plates). Detection of reactive oxygen species (ROS) in cells. Used in microscopy and staining techniques.

Its main purpose is to help visualize or detect substances through fluorescence. Scientists use it because it glows strongly under specific light allowing easy tracking and measurement of biological or chemical reactions.

2 7-Dichlorofluorescein is a fluorescent organic dye derived from fluorescein. It appears as a yellow solid and emits bright green fluorescence under light making it useful in laboratory detection methods.