3,3,5,5-Tetramethylbenzidine PurCert Analytical Standard for GC, >=98.0%

PurCert


Linear Formula:  C16H20N2
Molecular Weight:  240.34 g/mol
CAS Number: 54827-17-7

Properties

Water Solubility Insoluble
Assay Purity >=98%
M pt. 168-171 °C
Form Solid

Safety Information

Hazard Statement(s) H302,H351,H413
Precautionary Statement  P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
GHS Pictogram
Signal word Warning
HS Code 29215990
Storage Temp. Room Temperature
Storage Class 11 - Combustible Solids
UN Number NA

Description

Application
TMB is widely used in enzyme-linked immunosorbent assays (ELISAs) Western blotting immunohistochemistry and other diagnostic and research applications for the sensitive detection and quantification of HRP-labeled targets.

Purpose
The purpose of TMB is to detect and measure peroxidase enzyme activity particularly horseradish peroxidase (HRP) in biochemical and immunological assays.

General Description
its main functions is a highly sensitive chromogenic substrate for peroxidase enzymes particularly horseradish peroxidase (HRP). Upon oxidation in the presence of hydrogen peroxide and peroxidase TMB produces a blue-colored product that changes to yellow after the addition of an acid stop solution enabling easy visual or spectrophotometric detection.