Ammonium trifluoroacetate is commonly used in high-performance liquid chromatography (HPLC) liquid chromatographymass spectrometry (LCMS) peptide and protein analysis and other analytical chemistry applications because it is volatile and compatible with mass spectrometric detection.

Ammonium trifluoroacetate is primarily used as a volatile buffering agent to maintain a controlled pH in analytical and chemical processes.

Ammonium trifluoroacetate is an ammonium salt of trifluoroacetic acid that appears as a white crystalline powder and is highly soluble in water and polar organic solvents.