It is used orally in capsules tablets or suspensions to treat bacterial infections such as respiratory tract infections urinary tract infections skin infections otitis media and Helicobacter pylori eradication in combination therapy.

Amoxicillin trihydrate inhibits bacterial cell wall synthesis by binding penicillin-binding proteins leading to cell lysis and death effective against Gram-positive and some Gram-negative bacteria including Streptococcus Staphylococcus (non--lactamase

Amoxicillin trihydrate is the trihydrate form of the semisynthetic penicillin antibiotic amoxicillin with molecular formula CHNOS (MW 419.45 gmol CAS 61336-70-7) featuring a -lactam ring fused to a five-membered thiazolidine ring with a p-hydroxyphenylglycine side chain and gem-dimethyl groups at C-2 appearing as a white to off-white crystalline powder.