Its applications include treatment of bradycardia (slow heart rate) use as a pre-anesthetic to dry secretions and as an antidote in cases of organophosphate or nerve agent poisoning.

The purpose of atropine sulfate monohydrate is to counteract parasympathetic nervous system activity thereby restoring normal physiological function in conditions where excessive cholinergic stimulation occurs.

It is commonly used in medical settings to increase heart rate reduce excessive salivation and relax smooth muscles in organs like the intestines and lungs.