BMT activator (also known as 5-Benzylmercapto-1H-tetrazole or BTT)demonstrates important advantages in the syntheses of oligonucleotides1. Coupling efficiencies of 99 % with impeccable quality2. Lower percentage of n-1 sequences3. Dramatic reduction of coupling times to under 3 minutes in RNA syntheses4. Efficient RNA synthesis using 50 % or less monomer5. Excellent batch to batch consistency for reproducible and stable oligo production

BMT Crystal (5-benzylmercaptotetrazole) is used as a solid activator reagent for phosphoramidite chemistry particularly in the synthesis of DNA and RNA oligonucleotides.

Its purpose is to activate nucleoside phosphoramidites during the coupling step increasing their reactivity toward the free hydroxyl group of the growing oligonucleotide chain and thereby supporting efficient chain elongation.

BMT Crystal is the solid crystalline form of 5-benzylmercaptotetrazole an acidic activator commonly used in oligonucleotide synthesis. It can be dissolved in a suitable anhydrous solvent such as acetonitrile to prepare an activator solution at the required concentration.