Boc-Ile-OH is widely used as a building block in solution-phase and solid-phase peptide synthesis for the preparation of peptides peptide-based drugs and biochemical research compounds.

Its purpose is to protect the amino group of L-isoleucine during peptide synthesis enabling selective peptide bond formation and minimizing unwanted side reactions.

Boc-Ile-OH is the Boc (tert-butoxycarbonyl)-protected derivative of L-isoleucine in which the -amino group is protected while the carboxyl group remains free for peptide bond formation.