DMT-2-O-Me-rU Phosphoramidite is used as a building block in the automated solid-phase synthesis of RNA oligonucleotides containing 2-O-methyl uridine modifications.

Its purpose is to introduce a protected 2-O-methyl uridine residue into RNA sequences enhancing the oligonucleotides nuclease resistance stability and hybridization performance while reducing immunogenicity.

DMT-2-O-Me-rU Phosphoramidite is a chemically protected uridine phosphoramidite in which the 5-hydroxyl group is protected with a dimethoxytrityl (DMT) group and the 2-hydroxyl group is methylated making it suitable for efficient stepwise incorporation into RNA oligonucleotides during automated solid-phase synthesis.