DMT-2-O-Methyl-rC(Ac) Phosphoramidite is used in the solid-phase chemical synthesis of modified RNA oligonucleotides particularly those containing 2-O-methylcytidine residues.

The purpose of DMT-2-O-Methyl-rC(Ac) Phosphoramidite is to incorporate a protected 2-O-methylcytidine nucleotide into RNA sequences thereby enhancing the stability nuclease resistance and hybridization properties of the resulting oligonucleotides.

DMT-2-O-Methyl-rC(Ac) Phosphoramidite is a chemically protected cytidine phosphoramidite in which the 5-hydroxyl group is protected with a dimethoxytrityl (DMT) group the exocyclic amino group is protected with an acetyl (Ac) group and the 2-hydroxyl group is methylated making it suitable for automated solid-phase RNA oligonucleotide synthesis.