It helps in optimizing chromatographic conditions and verifying the accuracy and precision of FAME analytical methods.The known fatty acid profile allows it to be used as a reference to compare unknown samples.

The primary purpose of using menhaden fish oil in FAME analysis is to serve as a standard or test sample for the identification and quantification of fatty acids.

Menhaden fish oil is a marine oil rich in long-chain polyunsaturated fatty acids particularly omega-3 fatty acids such as EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid). It typically contains a complex mixture of saturated monounsaturated and polyunsaturated fatty acids making it an ideal reference material for analytical studies.