It is widely used as a building block in solid-phase peptide synthesis (SPPS) for incorporating D-serine residues into peptides with protected functional groups.

Its main purpose is to enable controlled peptide chain assembly by protecting reactive groups ensuring selective reactions and preventing unwanted side reactions during synthesis.

Fmoc-D-Ser(tBu)-OH is a fluorenylmethyloxycarbonyl (Fmoc) protected D-serine derivative in which the hydroxyl group is protected by a tert-butyl (tBu) group commonly used in peptide synthesis.