Used as a pH indicator in acidbase titrations. Applied in analytical and educational laboratories. Used in microbiology testing and chemical analysis. Sometimes used as a staining agent in research work.

Its main purpose is to undergo reversible protonation of the azo and dimethylamino groups producing sharp color changes for endpoint detection in weak base-strong acid titrations and bacterial glucose metabolism identification.

Methyl Red is a red-colored azo dye commonly used as an acidbase indicator in laboratories. It changes color depending on the pH of the solution.