It can be used in pharmaceutical and analytical research particularly for investigating nitrosation-related impurities and the characterization of folate-related compounds.

It is mainly used as a reference or research compound for studying the chemical modification degradation and nitrosation of folic acid.

N-Nitroso-folic acid is a nitroso derivative of folic acid containing a nitroso functional group. It is primarily encountered as a research compound rather than as a commonly used therapeutic ingredient.