Protease from Staphylococcus aureus V8 strain is primarily used as a research and laboratory reagent for controlled protein digestion particularly in proteomics and biochemical studies.

Its main purpose is to cleave proteins into smaller peptides at specific amino acid residues making it useful for protein identification peptide mapping sequence analysis and characterization of protein structure.

Protease from Staphylococcus aureus V8 strain commonly known as V8 protease or Glu-C protease is a serine protease that preferentially cleaves peptide bonds on the C-terminal side of glutamic acid residues and under certain conditions can also cleave after aspartic acid residues.