Thiazolyl Blue Tetrazolium Bromide

For R&D use onlynot for drug household or other uses.

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.MTT produces a yellowish solution that is converted to dark blue water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemicalcytochemical reagentand for the detection of NAD.ADP-linked enzyme systems in tissue cannot be detected with MTT due to binding of the cation by the cyanide trap used.MTT is rapidly reduced to the formazan which chelates with nickel copper and cobalt the cobalt chelate has been used in oxidative systems.