Biochanin A has been used to evaluate its effect on the ergotamine (ERT)-induced contractility in bovine mesenteric vasculature.[2]It may be used to test its anticancer potential in human glioblastoma cell lines.[3]It has been used as a reference standard in high-performance liquid chromatographytandem mass spectrometry.

For R&D use onlynot for drug household or other uses.

Biochanin A (BCA) is synthesizedin vitrofrom phloroglucinol. A series of steps involving FriedelCrafts reaction results in an intermediate product 1-(246-trihydroxyphenyl)-2-(4 methoxyphenyl) ethanone which post cyclization leads to BCA. It is catabolized to isoflavone genistein post ingestion.