As a chemiluminiscent substance. Used to measure opsonic and phagocytic function. As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency. Used as a forensic test for blood. Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminolperoxidase-based assay.

For R&D use onlynot for drug household or other uses.

Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide PAN hydrogen peroxide ozone and other atmospheric oxidants.