Gly-Gly has been used as a buffer for the measurement of glucose-6-phosphate dehydrogenase (G6PDH) activity and as a component of luciferase assay buffer.

For R&D use onlynot for drug household or other uses.

Glycylglycine is the dipeptide of glycine making it the simplest peptide. The compound was first synthesized by Emil Fischer and Ernest Fourneau in 1901 by boiling 25-diketopiperazine (glycine anhydride) with hydrochloric acid. Shaking with alkali and other synthesis methods have been reported. Because of its low toxicity it is useful as a buffer for biological systems with effective ranges between pH 2.5-3.8 and 7.5-8.9 however it is only moderately stable for storage once dissolved. It is used in the synthesis of more complex peptides.