27-Dibromo-9-N-Octylcarbazole 98.0%(GC)

Trypan blue has been used as a dye in trypan blue exclusion assaycell viability assay to detect dead cells. Trypan blue is a vital stain used to selectively colour dead tissues or cells blue. It is a diazo dye.

For R&D use onlynot for drug household or other uses.

Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane in a viable cell trypan blue is not absorbed however it traverses the membrane in a dead cell. Hence dead cells are shown as a distinctive blue colour under a microscope. Since live cells are excluded from staining this staining method is also described as a dye exclusion method. This dye may be a cause of certain birth defects such as encephalocele.